The G285S mutation in nsP1 is sufficient to render Sindbis virus as a stable vector for gene delivery
Neuroscience, gene therapy, and vaccine have all benefited from the increased use of viral vectors.Sindbis virus (SINV) is a notable candidate among these vectors.However, viral vectors commonly suffer from a loss of expression of the transgene, especially RNA viral vectors.In this study, we used a directed evolution approach by continuous passage